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- ArticlePollack ED, Leibig V.Science. 1977 Aug 26;197(4306):899-900.Limb bud mesenchyme enhances and directs the growth of tadpole spinal cord nerve fibers in tissue culture. This effect on elongating neurites may involve alterations in nerve-substratum interactions by the presence of undifferentiated target tissues. The relationship between nerve fibers and their potential innervation sites can explain directed nerve growth to the developing limb.